Genetic diversity and phylogenetic analysis of mayfly Caenis (Insecta: Ephemeroptera) using Cytochrome C Oxidase I (COI) and 12s rRNA genes from Thailand

Abstract. Prakrongrak N, Boonsoong B, Monthatong M. 2023. Genetic diversity and phylogenetic analysis of mayfly Caenis (Insecta: Ephemeroptera) using Cytochrome C Oxidase I (COI) 12s rRNA genes from Thailand. Biodiversitas 24: 1989-1997. Mayflies in genus Caenisis one the top fifth species richnessbelonged to Family Caenidae. The present study aimed use partial mitochondrial COxidase subunit nucleotide sequences for molecular identification, relationships mayflies Stephens, 1835 From a total 37 specimens, thirteen nymphs were morphologically identified into five species: nasuta Malzacher, C. Picea Kimmins, ulmeriana cornigera Kang Yang longiforcipata Malzacher. other 24 samples sp.1 sp.5. Partial COI all compared with BOLD GenBank identification. However, result showed only genus-level identification as greater than 80% similarity. For delimitation, interspecific genetic distance among these ranged 14.4 26.6% 7.36 22.4% (12s rRNA). Intraspecific divergence levels 0.0% 2.35%. ABGD both divided data 10 groups corresponding morphospecies Caenis. Caenis, also classified each well supported clusters. In addition, we report Thailand first time. trees indicate close relationship between longiforcipata, which is by their similar morphology.